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验证数据展示

  • IHC staining of human placenta using 83735-5-RR

    IHC staining of human placenta using 83735-5-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83735-5-RR (FOXK2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXK2 antibody included
  • IHC staining of human placenta using 83735-5-RR

    IHC staining of human placenta using 83735-5-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83735-5-RR (FOXK2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXK2 antibody included
  • IHC staining of human colon using 83735-5-RR

    IHC staining of human colon using 83735-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83735-5-RR (FOXK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXK2 antibody included
  • IHC staining of human stomach cancer using 83735-5-RR

    IHC staining of human stomach cancer using 83735-5-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83735-5-RR (FOXK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FOXK2 antibody included
  • IF Staining of A431 using 83735-5-RR

    IF Staining of A431 using 83735-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FOXK2 antibody (83735-5-RR, Clone: 240692D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A431 using 83735-5-RR

    IF Staining of A431 using 83735-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FOXK2 antibody (83735-5-RR, Clone: 240692D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of SH-SY5Y using 83735-5-RR

    FC experiment of SH-SY5Y using 83735-5-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Foxk2 Recombinant Antibody (83735-5-RR, Clone:240692D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HEK-293T using 83735-5-RR

    FC experiment of HEK-293T using 83735-5-RR

    1x10^6 HEK-293T cells were intracellularly stained with 0.25 ug Foxk2 Recombinant Antibody (83735-5-RR, Clone:240692D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

FOXK2 Recombinant monoclonal antibody

FOXK2 Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83735-5-RR

产品说明书

CloneNo. 240692D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

ILF1, ILF 1, ILF, Forkhead box protein K2, forkhead box K2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman placenta tissue, human stomach cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHEK-293T cells, SH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy FOXK2 Ready-To-Use IHC Kit. FOXK2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83735-5-RR targets FOXK2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33625

Product name: Recombinant human FOXK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 430-660 aa of NM_004514.3

Sequence: LSSQPVLITVQRQLPQAIKPVTYTVATPVTTSTSQPPVVQTVHVVHQIPAVSVTSVAGLAPANTYTVSGQAVVTPAAVLAPPKAEAQENGDHREVKVKVEPIPAIGHATLGTASRIIQTAQTTPVQTVTIVQQAPLGQHQLPIKTVTQNGTHVASVPTAVHGQVNNAAASPLHMLATHASASASLPTKRHNGDQPEQPELKRIKTEDGEGIVIALSVDTPPAAVREKGVQN*

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 forkhead box K2
别名 ILF1, ILF 1, ILF, Forkhead box protein K2, forkhead box K2
计算分子量69 kDa
GenBank蛋白编号NM_004514.3
基因名称 FOXK2
Gene ID (NCBI) 3607
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ01167
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for FOXK2 antibody 83735-5-RRDownload protocol
IHC protocol for FOXK2 antibody 83735-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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