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验证数据展示

  • WB analysis using 28301-1-AP

    WB analysis using 28301-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28301-1-AP (FOXJ2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HuH-7 using 28301-1-AP

    WB analysis of HuH-7 using 28301-1-AP

    WB result of FOXJ2 antibody (28301-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXJ2 transfected HuH-7 cells.

  • Featured Product
  • KD/KO验证

FOXJ2 Polyclonal antibody

FOXJ2 Polyclonal Antibody for WB, ELISA
Cat No. 28301-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

FHX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHuH-7 cells, U-87 MG cells, human placenta tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28301-1-AP targets FOXJ2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 FOXJ2 fusion protein Ag27345 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box J2
别名 FHX
计算分子量 574 aa, 62 kDa
观测分子量70 kDa
GenBank蛋白编号BC136305
基因名称 FOXJ2
Gene ID (NCBI) 55810
RRIDAB_3669651
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P0K8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fork head box J2 (FOXJ2) is a transcription factor discovered in mammals and other vertebrates in 2000, which belongs to the Fork head box (Fox) transcription factor family, which shares a conserved DNA-binding domain, known as Fork Head. FOXJ2 participates in the regulation of cell proliferation, differentiation, and migration by targeting downstream genes through its DNA binding domain, and plays fundamental roles in embryonic development, tumorigenesis, and the progression of certain cancers. The expression patterns of FOXJ2 have been reported to be aberrant in a variety of cancers, including breast cancer, extra-hepatic cholangiocarcinoma, nasopharyngeal carcinoma, glioma, and non-small cell lung cancer (PMID: 28769576, PMID: 33725831, PMID: 35908066). This antibody has been validated for knockdown.

实验方案

Product Specific Protocols
WB protocol for FOXJ2 antibody 28301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols