验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20268-1-AP targets FOXH1-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | forkhead box H1 |
| 别名 | FAST 1, Fast 2, FAST1, FAST2, forkhead box H1, Forkhead box protein H1, FOXH1, hFAST 1 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 40-44 kDa |
| GenBank蛋白编号 | NM_003923 |
| 基因名称 | FOXH1 |
| Gene ID (NCBI) | 8928 |
| RRID | AB_10694676 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75593 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FOXH1, also named as FAST1 and FAST2, is a transcriptional activator. FOXH1 recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. It is required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. It forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). The antibody is specific to FOXH1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXH1-Specific antibody 20268-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
