验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, Jurkat cells, RAW 264.7 cells, U-937 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24835-1-AP targets FOXD4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag19226 Product name: Recombinant human FOXD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-439 aa of BC136570 Sequence: ILQQQQRHQEEDCANGCAPTKGAVLGGHLSAASALLRYQAVAEGSGLTSLAAPLGGEGTSPVFLVSPTPSSLAESAGPS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | forkhead box D4 |
| 别名 | FKHL9, forkhead box D4, Forkhead box protein D4, Forkhead related protein FKHL9, FOXD4, FOXD4A, FREAC 5, FREAC5, Myeloid factor alpha |
| 计算分子量 | 439 aa, 47 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC136570 |
| 基因名称 | FOXD4 |
| Gene ID (NCBI) | 2298 |
| RRID | AB_2879750 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12950 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Forkhead box protein D4(FOXD4)also named as FKHL9 or Myeloid factor alpha is a 439 amino acid protein, which contains one fork head DNA-binding domain. FOXD4L6 localizes in the nucleus. FOXD4 as an embryonic transcriptional regulator involves in leukemogenesis. The molecular weight of FOXD4L6 is 47kDa, but our experiment always detected a 60-70kDa protein by western blot.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXD4 antibody 24835-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
