验证数据展示
经过测试的应用
| Positive WB detected in | BGC-823 cells, HGC-27 cells, SGC-7901 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34275-1-AP targets FOXD2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag41978 Product name: Recombinant human FOXD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 11-100 aa of NM_004474.4 Sequence: MSSESSPAALSEADADIDVVGGGSGGGELPARSGPRAPRDVLPHGHEPPAEEAEADLAEDEEESGGCSDGEPRALASRGAAAAAGSPGPG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | forkhead box D2 |
| 别名 | FKHL17, Forkhead box protein D2, Forkhead-related protein FKHL17, Forkhead-related transcription factor 9, FREAC9 |
| 计算分子量 | 49kDa，495aa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | NM_004474.4 |
| 基因名称 | FOXD2 |
| Gene ID (NCBI) | 2306 |
| RRID | AB_3743182 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O60548 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FOXD2, as one descendant of the FOX gene family, has been mentioned in many articles to show a high expression in several cancers. FOXD2 showed a high expression in gastric adenocarcinoma tissues and cells, and silencing FOXD2 could hinder gastric adenocarcinoma cell proliferation. In addition, FOXD2 could facilitate gastric adenocarcinoma cell proliferation by activating the expression of its downstream gene IQGAP3 and increasing intracellular Ca2+ concentration (PMID: 37724892).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXD2 antibody 34275-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
