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验证数据展示

  • WB analysis using 22474-1-AP (same clone as 22474-1-PBS)

    WB analysis using 22474-1-AP (same clone as 22474-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22474-1-AP (FOXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 22474-1-AP (same clone as 22474-1-PBS)

    IHC staining of human colon cancer using 22474-1-AP (same clone as 22474-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOXA2 antibody included
  • IHC staining of human colon cancer using 22474-1-AP (same clone as 22474-1-PBS)

    IHC staining of human colon cancer using 22474-1-AP (same clone as 22474-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOXA2 antibody included
  • IP experiment of HepG2 using 22474-1-AP (same clone as 22474-1-PBS)

    IP experiment of HepG2 using 22474-1-AP (same clone as 22474-1-PBS)

    IP result of anti-FOXA2 (IP:22474-1-AP, 4ug; Detection:22474-1-AP 1:500) with HepG2 cells lysate 1320 ug. This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

  • IF Staining of rat stomach using 22474-1-AP (same clone as 22474-1-PBS)

    IF Staining of rat stomach using 22474-1-AP (same clone as 22474-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat stomach tissue using FOXA2 antibody (22474-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FOXA2 Polyclonal antibody, PBS Only

FOXA2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 22474-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

forkhead box A2, Forkhead box protein A2, Hepatocyte nuclear factor 3-beta, HNF 3 beta, HNF 3B

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FOXA2 Ready-To-Use IHC Kit. FOXA2 primary antibody included.

产品信息

22474-1-PBS targets FOXA2 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18221

Product name: Recombinant human FOXA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-160 aa of BC011780

Sequence: MLGAVKMEGHEPSDWSSYYAEPEGYSSVSNMNAGLGMNGMNTYMSMSAAAMGSGSGNMSAGSMNMSSYVGAGMSPSLAGMSPGAGAMAGMGGSAGAAGVAGMGPHLSPSLSPLGGQAAGAMGGLAPYANMNSMSPMYGQAGLSRARDPKTYRRSYTHAKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box A2
别名 forkhead box A2, Forkhead box protein A2, Hepatocyte nuclear factor 3-beta, HNF 3 beta, HNF 3B
计算分子量 457 aa, 48 kDa
观测分子量 55-65 kDa
GenBank蛋白编号BC011780
基因名称 FOXA2
Gene ID (NCBI) 3170
RRIDAB_2879110
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y261
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.

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