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验证数据展示

  • WB analysis using 22474-1-AP (same clone as 22474-1-PBS)

    WB analysis using 22474-1-AP (same clone as 22474-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22474-1-AP (FOXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 22474-1-AP (same clone as 22474-1-PBS)

    IHC staining of human colon cancer using 22474-1-AP (same clone as 22474-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOXA2 antibody included
  • IHC staining of human colon cancer using 22474-1-AP (same clone as 22474-1-PBS)

    IHC staining of human colon cancer using 22474-1-AP (same clone as 22474-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22474-1-AP (FOXA2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FOXA2 antibody included
  • FC experiment of HepG2 using 22474-1-AP (same clone as 22474-1-PBS)

    FC experiment of HepG2 using 22474-1-AP (same clone as 22474-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FOXA2 Polyclonal antibody (22474-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 22474-1-AP (same clone as 22474-1-PBS)

    IP experiment of HepG2 using 22474-1-AP (same clone as 22474-1-PBS)

    IP result of anti-FOXA2 (IP:22474-1-AP, 4ug; Detection:22474-1-AP 1:500) with HepG2 cells lysate 1320 ug. This data was developed using the same antibody clone with 22474-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FOXA2 Polyclonal antibody, PBS Only

FOXA2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 22474-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

forkhead box A2, Forkhead box protein A2, Hepatocyte nuclear factor 3-beta, HNF 3 beta, HNF 3B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FOXA2 Ready-To-Use IHC Kit. FOXA2 primary antibody included.

产品信息

22474-1-PBS targets FOXA2 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18221

Product name: Recombinant human FOXA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-160 aa of BC011780

Sequence: MLGAVKMEGHEPSDWSSYYAEPEGYSSVSNMNAGLGMNGMNTYMSMSAAAMGSGSGNMSAGSMNMSSYVGAGMSPSLAGMSPGAGAMAGMGGSAGAAGVAGMGPHLSPSLSPLGGQAAGAMGGLAPYANMNSMSPMYGQAGLSRARDPKTYRRSYTHAKP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box A2
别名 forkhead box A2, Forkhead box protein A2, Hepatocyte nuclear factor 3-beta, HNF 3 beta, HNF 3B
计算分子量 457 aa, 48 kDa
观测分子量 55-65 kDa
GenBank蛋白编号BC011780
基因名称 FOXA2
Gene ID (NCBI) 3170
RRIDAB_2879110
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y261
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FOXA2 also named as HNF 3 beta or TCF3B is a 457 amino acid protein, which contains one fork-head DNA-binding domains. FOXA2 can Shuttle between the nucleus and cytoplasm in a CRM1-dependent manner. Phosphorylation on FOXA2 Thr-156 abolishes binding to target promoters and subsequent transcription activation upon INS stimulation. FOXA2 as a transcription factor involves in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. The molecular weight of FOXA2 is 48kDa, but the phosphorylated FOXA2 may be a 55-65kDa protein.

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