验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, A549 cells, Calu-1 cells |
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
12663-1-AP targets FOSL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag3344 Product name: Recombinant human FOSL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-271 aa of BC016648 Sequence: MFRDFGEPGPSSGNGGGYGGPAQPPAAAQAAQQKFHLVPSINTMSGSQELQWMVQPHFLGPSSYPRPLTYPQYSPPQPRPGVIRALGPPPGVRRRPCEQISPEEEERRRVRRERNKLAAAKCRNRRKELTDFLQAETDKLEDEKSGLQREIEELQKQKERLELVLEAHRPICKIPEGAKEGDTGSTSGTSSPPAPCRPVPCISLSPGPVLEPEALHTPTLMTTPSLTPFTPSLVFTYPSTPEPCASAHRKSSSSSGDPSSDPLGSPTLLAL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FOS-like antigen 1 |
| 别名 | FOS like antigen 1, Fos related antigen 1, Fos-related antigen 1, FRA, fra 1 |
| 计算分子量 | 271 aa, 29 kDa |
| 观测分子量 | 30-40 kDa |
| GenBank蛋白编号 | BC016648 |
| 基因名称 | FOSL1 |
| Gene ID (NCBI) | 8061 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P15407 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FOSL1 (FOS Like 1, AP-1 Transcription Factor Subunit) is a protein-coding gene that produces the Fos-related antigen 1 (FRA1) protein, a key component of the AP-1 transcription factor complex. It is heavily involved in regulating cell proliferation, differentiation, tumorigenesis, and metastasis, particularly in cancers like lung, pancreatic, and breast cancer. FOSL1 forms heterodimers with JUN family proteins to form the AP-1 complex, which binds to DNA to regulate gene expression. It is considered an oncologist in many solid tumors, driving metastasis, EMT, and resistance to chemotherapy and radiotherapy. High expression of FOSL1 is associated with poor prognosis and tumor aggressiveness. It is often found in the stroma of reproductive tissues, cartilage, and in tumor microenvironments.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FOSL1 antibody 12663-1-AP | Download protocol |
| WB protocol for FOSL1 antibody 12663-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
