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c-Fos Monoclonal antibody
c-Fos Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse and More (1)
Applications
FC, WB, ELISA and More (3)
Conjugate
Unconjugated
CloneNo.
1G2C5
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, HSC-T6 cells, Jurkat cells, U-937 cells, RAW 264.7 cells, K-562 cells, THP-1 cells, NIH/3T3 cells |
Positive FC detected in | HeLa cells |
c-Fos is an extremely short-lived intracellular protein that needs to be detected in fresh samples to prevent its degradation.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66590-1-Ig targets c-Fos in WB, IP, FC, IHC, CoIP, ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Applications | FC, WB, ELISA |
Cited Applications | WB, IP, IHC, CoIP |
Tested Reactivity | Human, rat, mouse |
Cited Reactivity | human, mouse |
Immunogen | c-Fos fusion protein Ag24340 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | FOS |
Synonyms | AP 1, C FOS, Cellular oncogene fos, c-Fos, FOS, G0S7, Proto oncogene c Fos |
Calculated Molecular Weight | 41 kDa |
Observed Molecular Weight | 55-60 kDa |
GenBank Accession Number | BC004490 |
Gene Symbol | FOS |
Gene ID (NCBI) | 2353 |
RRID | AB_2881950 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P01100 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for c-Fos antibody 66590-1-Ig | Download protocol |
FC protocol for c-Fos antibody 66590-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Theranostics KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.
| ||
Phytomedicine Gastrodin alleviates NTG-induced migraine-like pain via inhibiting succinate/HIF-1α/TRPM2 signaling pathway in trigeminal ganglion | ||
J Headache Pain SS-31 alleviated nociceptive responses and restored mitochondrial function in a headache mouse model via Sirt3/Pgc-1α positive feedback loop | ||
J Headache Pain PACAP6-38 improves nitroglycerin-induced central sensitization by modulating synaptic plasticity at the trigeminal nucleus caudalis in a male rat model of chronic migraine | ||
Cancer Lett MicroRNA-490-3P targets CDK1 and inhibits ovarian epithelial carcinoma tumorigenesis and progression. |