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验证数据展示

  • WB analysis using 28380-1-AP (same clone as 28380-1-PBS)

    WB analysis using 28380-1-AP (same clone as 28380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28380-1-AP (FNIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. . This data was developed using the same antibody clone with 28380-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 28380-1-AP (same clone as 28380-1-PBS)

    IF Staining of C2C12 using 28380-1-AP (same clone as 28380-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using FNIP1 antibody (28380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28380-1-PBS in a different storage buffer formulation.

FNIP1 Polyclonal antibody, PBS Only

FNIP1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28380-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FNIP1 Ready-To-Use IHC Kit. FNIP1 primary antibody included.

产品信息

28380-1-PBS targets FNIP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28976

Product name: Recombinant human FNIP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 163-250 aa of BC001956

Sequence: FTARTGSSICGSLNTLQDSLEFINQDNNTLKADNNTVINGLLGNIGLSQFCSPRRAFSEQGPLRLIRSASFFAVHSNPMDMPGRELNE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 folliculin interacting protein 1
别名
计算分子量 508 aa, 57 kDa
观测分子量 130-150 kDa
GenBank蛋白编号BC001956
基因名称 FNIP1
Gene ID (NCBI) 96459
RRIDAB_2881128
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TF40
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Folliculin-interacting protein 1 (FNIP1) forms a complex with the protein folliculin (FLCN), together acting as guanosine triphosphate (GTP)-activating protein (GAP) for RagC. The FNIP1-FLCN complex has emerged as an amino acid sensor to the mechanistic target of rapamycin complex 1 (mTORC1), involved in how amino acids control TFEB activation. AMPK phosphorylation of FNIP1 induces lysosomal and mitochondrial biogenesis (PMID: 37079666)

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