验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13632-1-AP targets FNDC4 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4550 Product name: Recombinant human FNDC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC032725 Sequence: MPSGCHSSPPSGLRGDMASLVPLSPYLSPTVLLLVSCDLGFVRADRPPSPVNVTVTHLRANSATVSWDVPEGNIVIGYSISQQRQNGPGQRVIREVNTTTRACALWGLAEDSDYTVQVRSIGLRGESPPGPRVHFRTLKGSDRLPSNSSSPGDITVEGLDGERPLQTGEVVIIVVVLLMWAAVIGLFCRQYDIIKDNDSNNNPKEKGKGPEQSPQGRPVGTRQKKSPSINTIDV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibronectin type III domain containing 4 |
| 别名 | Fibronectin type III domain-containing protein 4, Fibronectin type III repeat-containing protein 1, FRCP1, UNQ6389/PRO21134 |
| 计算分子量 | 234 aa, 25 kDa |
| 观测分子量 | 21 kDa, 36 kDa |
| GenBank蛋白编号 | BC032725 |
| 基因名称 | FNDC4 |
| Gene ID (NCBI) | 64838 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H6D8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FNDC4 is a member of the fibronectin type III domain family of proteins. It is a secreted factor consisting of a C-terminal hydrophobic domain, a type III fibronectin domain, and an N-terminal membrane signaling region. FNDC4 is expressed at the highest levels in the liver and brain, but its expression levels are lower in many other tissues, including the heart, skeletal muscle, and adipose tissue. The N-terminal of FNDC4 can be cleaved, releasing the extracellular portion of the protein. (PMID: 32881638; 27066907)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FNDC4 antibody 13632-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
