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验证数据展示

  • WB analysis using 86272-1-RR (same clone as 86272-1-PBS)

    WB analysis using 86272-1-RR (same clone as 86272-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86272-1-RR (FNDC3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86272-1-PBS in a different storage buffer formulation.

  • Sample test of MP02305-1

    Sample test of MP02305-1

    The mean FNDC3B concentration was determined to be 70.0 ng/mL in HepG2 cell extract based on a 0.9 mg/mL extract load.

  • Cytometric bead array standard curve of MP02305-1

    Cytometric bead array standard curve of MP02305-1

    Cytometric bead array standard curve of MP02305-1, FNDC3B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86272-2-PBS. Detection antibody: 86272-1-PBS. Standard: Ag18357. Range: 3.125-200 ng/mL.

FNDC3B Recombinant antibody, PBS Only (Detector)

FNDC3B Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 86272-1-PBS

产品说明书

CloneNo. 250827H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Indirect ELISA, Sample test

别名

FAD104, fibronectin type III domain containing 3B, Fibronectin type III domain-containing protein 3B, HCV NS5A-binding protein 37, UNQ2421/PRO4979/PRO34274

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86272-1-PBS targets FNDC3B as part of a matched antibody pair:

MP02305-1: 86272-2-PBS capture and 86272-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 FNDC3B fusion protein Ag18357 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fibronectin type III domain containing 3B
别名 FAD104, fibronectin type III domain containing 3B, Fibronectin type III domain-containing protein 3B, HCV NS5A-binding protein 37, UNQ2421/PRO4979/PRO34274
计算分子量 1204 aa, 133 kDa
观测分子量 150 kDa
GenBank蛋白编号BC039297
基因名称 FNDC3B
Gene ID (NCBI) 64778
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ53EP0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibronectin type III domain-containing protein 3B(FNDC3B), also named FAD104, belongs to the FNDC3 family. NDC3B is a positive regulator of adipocyte differentiation and is expressed at an early stage of adipocyte differentiation. FNDC3B is an endoplasmic reticulum transmembrane protein with a single transmembrane domain at the C terminus preceded by nine repeated fibronectin type III domains(PMID: 32966780). Due to the ability of the fibronectin type III domain to bind to a variety of proteins, FNDC3B plays a crucial role in cell adhesion, proliferation, and growth signaling. FNDC3B is abnormally expressed in a variety of human cancers, including hepatocellular carcinoma, acute myeloid leukemia, colorectal carcinoma, and cervical carcinoma(PMID: 36275754).