验证数据展示
经过测试的应用
| Positive WB detected in | 4T1 cells, HL-60 cells, HepG2 cells, C6 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22605-1-AP targets FNDC3B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | FNDC3B fusion protein Ag18357 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibronectin type III domain containing 3B |
| 别名 | Factor for adipocyte differentiation 104, FAD104, fibronectin type III domain containing 3B, FNDC3B, HCV NS5A binding protein 37 |
| 计算分子量 | 1204 aa, 133 kDa |
| 观测分子量 | 150 kDa, 70 kDa |
| GenBank蛋白编号 | BC039297 |
| 基因名称 | FNDC3B |
| Gene ID (NCBI) | 64778 |
| RRID | AB_2879133 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q53EP0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FNDC3B antibody 22605-1-AP | Download protocol |
| IHC protocol for FNDC3B antibody 22605-1-AP | Download protocol |
| IP protocol for FNDC3B antibody 22605-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Sci FNDC3B 3'-UTR shortening escapes from microRNA-mediated gene repression and promotes nasopharyngeal carcinoma progression.
| ||
J Cell Physiol FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E-cadherin and CD44 expression. | ||
Am J Transl Res EYA1 promotes cell migration and tumor metastasis in hepatocellular carcinoma. | ||
J Cell Commun Signal CircRNA hsa_circ_0001627 aggravates cervical cancer progression through upregulation of FNDC3B and activating PI3K/mTOR signaling pathway |
