验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55055-1-AP targets Toca 1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | formin binding protein 1-like |
| 别名 | C1orf39, FNBP1L, formin binding protein 1 like, Toca 1, TOCA1 |
| 计算分子量 | 70 kDa |
| 观测分子量 | 63-75 kDa |
| GenBank蛋白编号 | NM_017737 |
| 基因名称 | Toca 1 |
| Gene ID (NCBI) | 54874 |
| RRID | AB_10858792 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5T0N5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Toca-1, also named as FNBP1L or C1orf39, belongs to the FNBP1 family. It is required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Toca-1 may bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. The antibody is specific to Toca-1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Toca 1 antibody 55055-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |