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验证数据展示

  • WB analysis using 16312-1-AP

    WB analysis using 16312-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16312-1-AP (FN3KRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 16312-1-AP

    WB analysis of HEK-293 using 16312-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16312-1-AP (FN3KRP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16312-1-AP

    IF Staining of HeLa using 16312-1-AP

    Immunofluorescent analysis of HeLa cells using 16312-1-AP (FN3KRP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 16312-1-AP

    IHC staining of mouse brain using 16312-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16312-1-AP (FN3KRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 16312-1-AP

    IHC staining of mouse brain using 16312-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16312-1-AP (FN3KRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FN3KRP Polyclonal antibody

FN3KRP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16312-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:2.7.1.-, EC:2.7.1.172, FN3K-related protein, FN3K-RP, Fructosamine-3-kinase-related protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16312-1-AP targets FN3KRP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9347

Product name: Recombinant human FN3KRP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-309 aa of BC014408

Sequence: MEELLRRELGCSSVRATGHSGGGCISQGRSYDTDQGRVFVKVNPKAEARRMFEGEMASLTAILKTNTVKVPKPIKVLDAPGGGSVLVMEHMDMRHLSSHAAKLGAQLADLHLDNKKLGEMRLKEAGTVGRGGGQEERPFVARFGFDVVTCCGYLPQVNDWQEDWVVFYARQRIQPQMDMVEKESGDREALQLWSALQLKIPDLFRDLEIIPALLHGDLWGGNVAEDSSGPVIFDPASFYGHSEYELAIAGMFGGFSSSFYSAYHGKIPKAPGFEKRLQLYQLFHYLNHWNHFGSGYRGSSLNIMRNLVK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fructosamine 3 kinase related protein
别名 EC:2.7.1.-, EC:2.7.1.172, FN3K-related protein, FN3K-RP, Fructosamine-3-kinase-related protein
计算分子量 309 aa, 34 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC014408
基因名称 FN3KRP
Gene ID (NCBI) 79672
RRIDAB_2105986
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HA64
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fn3KRP (Fn3K-related protein) are believed to be the first examples of deglycating enzymes acting on the Amadori product to reverse the early stages of the Maillard reaction in vivo in higher organisms (PMID: 17337043). Fn3KRP possesses an ATP-binding domain that is structurally related to that of both the aminoglycoside kinases and eukaryotic protein kinases. Fn3KRP possesses an intrinsic activity towards ketosamine substrates (PMID: 18637789). Catalog#16312-1-AP is a rabbit polyclonal antibody raised against the full-length of human FN3KRP. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein.

实验方案

Product Specific Protocols
WB protocol for FN3KRP antibody 16312-1-APDownload protocol
IHC protocol for FN3KRP antibody 16312-1-APDownload protocol
IF protocol for FN3KRP antibody 16312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols