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验证数据展示

  • WB analysis using 87803-1-RR (same clone as 87803-1-PBS)

    WB analysis using 87803-1-RR (same clone as 87803-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87803-1-RR (FMR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87803-1-PBS in a different storage buffer formulation.

  • WB analysis using 87803-1-RR (same clone as 87803-1-PBS)

    WB analysis using 87803-1-RR (same clone as 87803-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87803-1-RR (FMR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87803-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 87803-1-RR (same clone as 87803-1-PBS)

    IHC staining of human prostate cancer using 87803-1-RR (same clone as 87803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 87803-1-RR (FMR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87803-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FMR1 antibody included
  • IHC staining of human prostate cancer using 87803-1-RR (same clone as 87803-1-PBS)

    IHC staining of human prostate cancer using 87803-1-RR (same clone as 87803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 87803-1-RR (FMR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87803-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FMR1 antibody included
  • IF Staining of HeLa using 87803-1-RR (same clone as 87803-1-PBS)

    IF Staining of HeLa using 87803-1-RR (same clone as 87803-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FMR1 antibody (87803-1-RR, Clone: 253415C12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87803-1-PBS in a different storage buffer formulation.

FMR1 Recombinant monoclonal antibody, PBS Only

FMR1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87803-1-PBS

产品说明书

CloneNo. 253415C12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FMRP, fragile X mental retardation 1, Fragile X messenger ribonucleoprotein, Fragile X messenger ribonucleoprotein 1, FRAXA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FMR1 Ready-To-Use IHC Kit. FMR1 primary antibody included.

产品信息

87803-1-PBS targets FMR1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4697

Product name: Recombinant human FMR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-297 aa of BC038998

Sequence: MEELVVEVRGSNGAFYKAFVKDVHEDSITVAFENNWQPDRQIPFHDVRFPPPVGYNKDINESDEVEVYSRANEKEPCCWWLAKVRMIKGEFYVIEYAACDATYNEIVTIERLRSVNPNKPATKDTFHKIKLDVPEDLRQMCAKEAAHKDFKKAVGAFSVTYDPENYQLVILSINEVTSKRAHMLIDMHFRSLRTKLSLIMRNEEASKQLESSRQLASRFHEQFIVREDLMGLAIGTHGANIQQARKVPGVTAIDLDEDTCTFHIYGEDQDAVKKARSFLEFAEDVIQVPRNLVGLKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fragile X mental retardation 1
别名 FMRP, fragile X mental retardation 1, Fragile X messenger ribonucleoprotein, Fragile X messenger ribonucleoprotein 1, FRAXA
计算分子量 632 aa, 71 kDa
观测分子量 59-72 kDa
GenBank蛋白编号BC038998
基因名称 FMR1
Gene ID (NCBI) 2332
RRIDAB_3745759
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ06787
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

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