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Fibromodulin Polyclonal antibody

Fibromodulin Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 13281-1-AP

Print datasheet

Synonyms

fibromodulin, FM, FMOD, KSPG fibromodulin, SLRR2E

验证数据展示

  • WB analysis of human liver using 13281-1-AP

    WB analysis of human liver using 13281-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13281-1-AP (Fibromodulin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 13281-1-AP

    IF Staining of HeLa using 13281-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13281-1-AP (Fibromodulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman liver tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13281-1-AP targets Fibromodulin in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Fibromodulin fusion protein Ag4030 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibromodulin
Synonyms fibromodulin, FM, FMOD, KSPG fibromodulin, SLRR2E
Calculated Molecular Weight 376 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC035281
Gene Symbol FMOD
Gene ID (NCBI) 2331
RRIDAB_2105531
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06828
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibromodulin (FMOD), an extracellular matrix protein, belongs to the small leucine-rich proteoglycan family. Fibromodulin is a collagen-binding protein present in many types of connective tissues, e.g. cartilage, tendon, skin, sclera and cornea (PMID: 2531085). It is involved in fibrillogenesis, cell adhesion, and cytokine activity modulation (PMID: 15741214). Fibromodulin may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. The calculated molecular mass of fibromodulin is 43 kDa, extracellular fibromodulin appears as one single band of 59 kDa due to glycosylation (PMID: 2531085; 22138190).

实验方案

Product Specific Protocols
WB protocol for Fibromodulin antibody 13281-1-APDownload protocol
IF protocol for Fibromodulin antibody 13281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Fibromodulin overexpression drives oral squamous cell carcinoma via activating downstream EGFR signaling

Authors - Lingyun Xia
View Article