验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, mouse liver tissue, DU 145 cells, LNCaP cells, VCaP cells, RAW 264.7 cells, MCF-7 cells, rat liver tissue |
| Positive IHC detected in | human liver tissue, mouse liver tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16864-1-AP targets FMO5-specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | flavin containing monooxygenase 5 |
| 别名 | FMO5, Baeyer-Villiger monooxygenase 1, Dimethylaniline monooxygenase [N-oxide-forming] 5, EC:1.14.13.-, EC:1.14.13.8 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | NM_001461 |
| 基因名称 | FMO5 |
| Gene ID (NCBI) | 2330 |
| RRID | AB_11125955 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49326 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Microsomal flavin-containing monooxygenases (FMOs) [dimethylaniline monooxygenase (N-oxide forming) catalyze the FAD-, NADPH- and O2-dependent oxidation of a large number of structurally diverse compounds, including drugs, pesticides, and industrial chemicals containing a soft nucleophile(PMID:12488558 ). FMO5, which belongs to the FMO family, is a lesser component of human liver microsomes and is present at about one-third the level of FMO3. FMO5 protein is also present at very low levels in kidney, however, FMO5 exhibits a severely restricted substrate specificity for most drugs and other xenobiotics examined to date(PMID:10950857). It has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FMO5-specific antibody 16864-1-AP | Download protocol |
| IHC protocol for FMO5-specific antibody 16864-1-AP | Download protocol |
| WB protocol for FMO5-specific antibody 16864-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Drug Metab Dispos Identification of flavin-containing monooxygenase 5 (FMO5) as a regulator of glucose homeostasis and a potential sensor of gut bacteria.
| ||
J Transl Med Anthocyanin improves kidney function in diabetic kidney disease by regulating amino acid metabolism |
