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验证数据展示

  • WB analysis using 67881-1-Ig

    WB analysis using 67881-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67881-1-Ig (FMO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of mouse liver using 67881-1-Ig

    IF Staining of mouse liver using 67881-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse liver using 67881-1-Ig

    IF Staining of mouse liver using 67881-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using FMO4 antibody (67881-1-Ig, Clone: 2D12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HuH-7 using 67881-1-Ig

    FC experiment of HuH-7 using 67881-1-Ig

    1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human FMO4 (67881-1-Ig, Clone:2D12E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

FMO4 Monoclonal antibody

FMO4 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA
Cat No. 67881-1-Ig

产品说明书

CloneNo. 2D12E10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF-P, FC (Intra), ELISA

别名

2D12E10, Dimethylaniline monooxygenase [N-oxide-forming] 4, EC:1.14.13.8, FMO2, Hepatic flavin-containing monooxygenase 4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inrabbit liver tissue, HuH-7 cells, pig liver tissue, rat liver tissue, mouse liver tissue
Positive IF-P detected inmouse liver tissue
Positive FC (Intra) detected inHuH-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67881-1-Ig targets FMO4 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag7935

Product name: Recombinant human FMO4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 24-354 aa of BC002780

Sequence: EDLEPTCFERSDDIGGLWKFTESSKDGMTRVYKSLVTNVCKEMSCYSDFPFHEDYPNFMNHEKFWDYLQEFAEHFDLLKYIQFKTTVCSITKRPDFSETGQWDVVTETEGKQNRAVFDAVMVCTGHFLNPHLPLEAFPGIHKFKGQILHSQEYKIPEGFQGKRVLVIGLGNTGGDIAVELSRTAAQVLLSTRTGTWVLGRSSDWGYPYNMMVTRRCCSFIAQVLPSRFLNWIQERKLNKRFNHEDYGLSITKGKKAKFIVNDELPNCILCGAITMKTSVIEFTETSAVFEDGTVEENIDVVIFTTGYTFSFPFFEEPLKSLCTKKIFLYKQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 flavin containing monooxygenase 4
别名 2D12E10, Dimethylaniline monooxygenase [N-oxide-forming] 4, EC:1.14.13.8, FMO2, Hepatic flavin-containing monooxygenase 4
计算分子量 63 kDa
观测分子量63 kDa
GenBank蛋白编号BC002780
基因名称 FMO4
Gene ID (NCBI) 2329
RRIDAB_2918638
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP31512
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for FMO4 antibody 67881-1-IgDownload protocol
IF protocol for FMO4 antibody 67881-1-IgDownload protocol
WB protocol for FMO4 antibody 67881-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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