验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HepG2 cells, human placenta tissue, K-562 cells, mouse kidney tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human breast cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26841-1-AP targets FLVCR1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag25220 Product name: Recombinant human FLVCR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-107 aa of BC048312 Sequence: MARPDDEEGAAVAPGHPLAKGYLPLPRGAPVGKESVELQNGPKAGTFPVNGPPRDSLAAASGVLGGPQTPLAPEEETQARLLPAGAGAETPGAESSPLPLTALSPRR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | feline leukemia virus subgroup C cellular receptor 1 |
| 别名 | Feline leukemia virus subgroup C receptor-related protein 1, FLVCR, Heme transporter FLVCR1 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 60 kDa~70 kDa |
| GenBank蛋白编号 | BC048312 |
| 基因名称 | FLVCR1 |
| Gene ID (NCBI) | 28982 |
| RRID | AB_2880654 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5Y0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FLVCR1(feline leukemia virus subgroup C receptor 1) has been reported to have a crucial role in a variety of biological processes, including cell proliferation, cell death, apoptosis, oxidative stress response, cellular differentiation, and metabolism (PMID: 29532854). In addition, FLVCR1 can be applied as a clinical prognostic marker for patients with ESCC (PMID: 33842377).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FLVCR1 antibody 26841-1-AP | Download protocol |
| IHC protocol for FLVCR1 antibody 26841-1-AP | Download protocol |
| IP protocol for FLVCR1 antibody 26841-1-AP | Download protocol |
| WB protocol for FLVCR1 antibody 26841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Heme allocation in eukaryotic cells relies on mitochondrial heme export through FLVCR1b to cytosolic GAPDH. | ||
J Nanobiotechnology Apoptotic metabolites ameliorate bone aging phenotypes via TCOF1/FLVCR1-mediated mitochondrial homeostasis
| ||
Cell Rep Med Dysregulation of FLVCR1a-dependent mitochondrial calcium handling in neural progenitors causes congenital hydrocephalus
| ||
Cell Rep Flvcr1a deficiency promotes heme-based energy metabolism dysfunction in skeletal muscle | ||
Clin Exp Ophthalmol FLVCR1 Deficiency Impairs Mitochondrial Homeostasis in Retinal Degeneration: Choline as a Potential Therapy.
| ||
Biochim Biophys Acta Mol Basis Dis The role of FLVCR1 and FLVCR2 in choline transport in the Caco-2 intestinal epithelial cell model and rat small intestine |
