验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, L02 cells |
| Positive IF/ICC detected in | L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
18073-1-AP targets FKBP9 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | FKBP9 fusion protein Ag12715 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | FK506 binding protein 9, 63 kDa |
| 别名 | FK506-binding protein 9, FK506 binding protein 9, EC:5.2.1.8, 63 kDa FKBP, 63 kDa FK506-binding protein |
| 计算分子量 | 570 aa, 63 kDa |
| 观测分子量 | 63 kDa |
| GenBank蛋白编号 | BC101723 |
| 基因名称 | FKBP9 |
| Gene ID (NCBI) | 11328 |
| RRID | AB_2878490 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95302 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FKBP9 (FK506-binding protein 9, also referred to as FKBP60 or FKBP63) belongs to a family of immunophilins that are able to bind to FK506, an immunosuppressive drug. Several members of the FKBP family, including FKBP9, FKBP13, FKBP23 and FKBP65, localize to the endoplasmic reticulum (ER) as they contain the ER retention motif H/R/KDEL (PMID:32111229). In addition, the FKBP9 mutation was also reported to be associated with disease-free survival rates in pheochromocytoma or paraganglioma (PMID:28386672).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FKBP9 antibody 18073-1-AP | Download protocol |
| IF protocol for FKBP9 antibody 18073-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
