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验证数据展示

  • WB analysis of human lung using 12092-1-AP

    WB analysis of human lung using 12092-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12092-1-AP (FKBP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 12092-1-AP

    IHC staining of human liver cancer using 12092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12092-1-AP (FKBP7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 12092-1-AP

    IHC staining of human liver cancer using 12092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12092-1-AP (FKBP7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FKBP7 Polyclonal antibody

FKBP7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12092-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

23 kDa FK506 binding protein, 23 kDa FKBP, FK506 binding protein 7, FKBP 23, FKBP 7, FKBP23, FKBP7, PPIase, PPIase FKBP7, Rotamase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman lung tissue, human heart tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12092-1-AP targets FKBP7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FKBP7 fusion protein Ag2733 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FK506 binding protein 7
别名 23 kDa FK506 binding protein, 23 kDa FKBP, FK506 binding protein 7, FKBP 23, FKBP 7, FKBP23, FKBP7, PPIase, PPIase FKBP7, Rotamase
计算分子量 259 aa, 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC009711
基因名称 FKBP7
Gene ID (NCBI) 51661
RRIDAB_2103418
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y680
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FKBP7(FK506-binding protein 7) is also named as FKBP23 and belongs to immunophilin class of proteins family. This protein contains an N-terminal hydrophobic signal sequence, a PPIase motif, 2 EF-hand domains, and 2 putative N-glycosylation sites. The calculated molecular mass is about 25 kD, but becomes 33 kD following cleavage of the signal sequence(PMID:9806833). It could bind to BiP in ER in a process regulated by the concentration of Ca2+(PMID:22269648). It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for FKBP7 antibody 12092-1-APDownload protocol
IHC protocol for FKBP7 antibody 12092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols