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验证数据展示

  • WB analysis of PC-3 using 22623-1-AP

    WB analysis of PC-3 using 22623-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 22623-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 22623-1-AP

    IHC staining of human colon using 22623-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22623-1-AP (FKBP52 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FKBP4/FKBP52 antibody included
  • IHC staining of human colon using 22623-1-AP

    IHC staining of human colon using 22623-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22623-1-AP (FKBP52 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FKBP4/FKBP52 antibody included
  • IF Staining of PC-3 using 22623-1-AP

    IF Staining of PC-3 using 22623-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using FKBP52 antibody (22623-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of PC-3 using 22623-1-AP

    IF Staining of PC-3 using 22623-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 22623-1-AP (FKBP52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

FKBP52 Polyclonal antibody

FKBP52 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22623-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

FKBP4, FKBP-4, FK506-binding protein 4, EC:5.2.1.8, 52 kDa FK506-binding protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy FKBP4/FKBP52 Ready-To-Use IHC Kit. FKBP4/FKBP52 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22623-1-AP targets FKBP52 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18704

Product name: Recombinant human FKBP52 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-286 aa of BC007924

Sequence: MTAEEMKATESGAQSAPLPMEGVDISPKQDEGVLKVIKREGTGTEMPMIGDRVFVHYTGWLLDGTKFDSSLDRKDKFSFDLGKGEVIKAWDIAIATMKVGEVCHITCKPEYAYGSAGSPPKIPPNATLVFEVELFEFKGEDLTEEEDGGIIRRIQTRGEGYAKPNEGAIVEVALEGYYKDKLFDQRELRFEIGEGENLDLPYGLERAIQRMEKGEHSIVYLKPSYAFGSVGKEKFQIPPNAELKYELHLKSFEKAKESWEMNSEEKLEQSTIVKERGTVYFKEGKY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FK506 binding protein 4, 59kDa
别名 FKBP4, FKBP-4, FK506-binding protein 4, EC:5.2.1.8, 52 kDa FK506-binding protein
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC007924
基因名称 FKBP52
Gene ID (NCBI) 2288
RRIDAB_2879136
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ02790
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The large molecular-weight immunophilin, FKBP52, is a known target of the immunosuppressive drug FK506. FKBP52 exhibits peptidyl-prolyl cis-trans isomerase (PPIase) activity, which is inhibited by the binding of FK506-properties that it shares with the smaller but better-studied immunophilin, FKBP12(PMID:15381148). It is essential to AR-mediated induction of genes involved in sexually dimorphic development of the penis (NKX3-1), in erectile function of the mature organ (NOS2 and NOS3), and in promotion of apoptosis during development(PMID:20605780).

实验方案

Product Specific Protocols
IF protocol for FKBP52 antibody 22623-1-APDownload protocol
IHC protocol for FKBP52 antibody 22623-1-APDownload protocol
WB protocol for FKBP52 antibody 22623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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