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  • Featured Product
  • KD/KO Validated

FKBP3 Polyclonal antibody

FKBP3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 11755-1-AP

Print datasheet

Synonyms

25 kDa FK506 binding protein, 25 kDa FKBP, FK506 binding protein 3, FK506 binding protein 3, 25kDa, FKBP 25, FKBP 3, FKBP25, FKBP3, Immunophilin FKBP25, PPIase, PPIase FKBP3, Rotamase

验证数据展示

  • WB analysis of HeLa using 11755-1-AP

    WB analysis of HeLa using 11755-1-AP

    WB result of FKBP3 antibody (11755-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP3 transfected HeLa cells.

  • WB analysis of PC-3 using 11755-1-AP

    WB analysis of PC-3 using 11755-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 11755-1-AP

    IHC staining of mouse brain using 11755-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11755-1-AP

    IHC staining of mouse brain using 11755-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 11755-1-AP

    IF Staining of HeLa using 11755-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11755-1-AP (FKBP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells, A431 cells
Positive IHC detected inmouse brain tissue, human lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11755-1-AP targets FKBP3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen FKBP3 fusion protein Ag2351 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FK506 binding protein 3, 25kDa
Synonyms 25 kDa FK506 binding protein, 25 kDa FKBP, FK506 binding protein 3, FK506 binding protein 3, 25kDa, FKBP 25, FKBP 3, FKBP25, FKBP3, Immunophilin FKBP25, PPIase, PPIase FKBP3, Rotamase
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC020809
Gene Symbol FKBP3
Gene ID (NCBI) 2287
RRIDAB_2102983
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00688
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FKBP3 antibody 11755-1-APDownload protocol
IHC protocol for FKBP3 antibody 11755-1-APDownload protocol
IF protocol for FKBP3 antibody 11755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

FKBP3 aggravates the malignant phenotype of diffuse large B-cell lymphoma by PARK7-mediated activation of Wnt/β-catenin signalling

Authors - Xiaojing Xing
  • KD Validated
View Article
humanIHC

BMC Cancer

Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma.

Authors - Yang Zhai
View Article