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验证数据展示

  • WB analysis of HeLa using 11755-1-AP

    WB analysis of HeLa using 11755-1-AP

    WB result of FKBP3 antibody (11755-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP3 transfected HeLa cells.

  • WB analysis of PC-3 using 11755-1-AP

    WB analysis of PC-3 using 11755-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 11755-1-AP

    IHC staining of mouse brain using 11755-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11755-1-AP

    IHC staining of mouse brain using 11755-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 11755-1-AP

    IF Staining of HeLa using 11755-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FKBP3 antibody (11755-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HepG2 using 11755-1-AP

    IF Staining of HepG2 using 11755-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FKBP3 antibody (11755-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

FKBP3 Polyclonal antibody

FKBP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11755-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

FKBP25, FKBP 3, FKBP 25, FK506-binding protein 3, EC:5.2.1.8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells, A431 cells
Positive IHC detected inmouse brain tissue, human lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11755-1-AP targets FKBP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag2351

Product name: Recombinant human FKBP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-224 aa of BC020809

Sequence: MAAAVPQRAWTVEQLRSEQLPKKDIIKFLQEHGSDSFLAEHKLLGNIKNVAKTANKDHLVTAYNHLFETKRFKGTESISKVSEQVKNVKLNEDKPKETKSEETLDEGPPKYTKSVLKKGDKTNFPKKGDVVHCWYTGTLQDGTVFDTNIQTSAKKKKNAKPLSFKVGVGKVIRGWDEALLTMSKGEKARLEIEPEWAYGKKGQPDAKIPPNAKLTFEVELVDID

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FK506 binding protein 3, 25kDa
别名 FKBP25, FKBP 3, FKBP 25, FK506-binding protein 3, EC:5.2.1.8
计算分子量 224 aa, 25 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC020809
基因名称 FKBP3
Gene ID (NCBI) 2287
RRIDAB_2102983
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ00688
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FKBP3 antibody 11755-1-APDownload protocol
IHC protocol for FKBP3 antibody 11755-1-APDownload protocol
IF protocol for FKBP3 antibody 11755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

FKBP3 aggravates the malignant phenotype of diffuse large B-cell lymphoma by PARK7-mediated activation of Wnt/β-catenin signalling

Authors - Xiaojing Xing
  • KD Validated
View Article
humanIHC

BMC Cancer

Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma.

Authors - Yang Zhai
View Article