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FIS1 Monoclonal antibody, PBS Only

FIS1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2F10E1

Cat No : 66635-1-PBS

Print datasheet

Synonyms

CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11

验证数据展示

  • WB analysis using 66635-1-Ig (same clone as 66635-1-PBS)

    WB analysis using 66635-1-Ig (same clone as 66635-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66635-1-Ig (FIS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66635-1-Ig (same clone as 66635-1-PBS)

    IHC staining of human breast cancer using 66635-1-Ig (same clone as 66635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66635-1-Ig (same clone as 66635-1-PBS)

    IF Staining of HepG2 using 66635-1-Ig (same clone as 66635-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66635-1-Ig (FIS1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

  • WB analysis using 66635-1-Ig (same clone as 66635-1-PBS)

    WB analysis using 66635-1-Ig (same clone as 66635-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66635-1-Ig (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

产品信息

66635-1-PBS targets FIS1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity Human
Immunogen FIS1 fusion protein Ag1409 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Synonyms CGI 135, FIS1, FIS1 homolog, hFis1, TPR repeat protein 11, TTC11
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC009428
Gene Symbol FIS1
Gene ID (NCBI) 51024
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y3D6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (21257639, 21459773, 23333625)