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验证数据展示

  • IHC staining of human ovary cancer using 83863-3-RR

    IHC staining of human ovary cancer using 83863-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83863-3-RR (FIP1L1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FIP1L1 antibody included
  • IF Staining of MCF-7 using 83863-3-RR

    IF Staining of MCF-7 using 83863-3-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FIP1L1 antibody (83863-3-RR, Clone: 240658A8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A549 using 83863-3-RR

    FC experiment of A549 using 83863-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-3-RR, Clone:240658A8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • FC experiment of U-2 OS using 83863-3-RR

    FC experiment of U-2 OS using 83863-3-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-3-RR, Clone:240658A8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83863-3-RR

    Affinity and Kinetic Characterization of 83863-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83863-3-RR against Human FIP1L1 were performed. The affinity constant is 1.63 nM.

FIP1L1 Recombinant antibody

FIP1L1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83863-3-RR

产品说明书

CloneNo. 240658A8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

240658A8, FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA549 cells, U2OS cells
Planning an IHC experiment? We recommend our IHCeasy FIP1L1 Ready-To-Use IHC Kit. FIP1L1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83863-3-RR targets FIP1L1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 FIP1L1 fusion protein Ag2921 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 FIP1 like 1 (S. cerevisiae)
别名 240658A8, FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE
计算分子量 594 aa, 66 kDa
GenBank蛋白编号BC017724
基因名称 FIP1L1
Gene ID (NCBI) 81608
RRIDAB_3671449
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ6UN15
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).

实验方案

Product Specific Protocols
IHC protocol for FIP1L1 antibody 83863-3-RRDownload protocol
IF protocol for FIP1L1 antibody 83863-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols