• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FIP1L1 Polyclonal antibody

FIP1L1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12276-1-AP

Print datasheet

Synonyms

Factor interacting with PAP, FIP1, FIP1 like 1 (S. cerevisiae), FIP1 like 1 protein, FIP1L1, FIP1L1 PDGFRalpha, hFip1, Rhe

验证数据展示

  • WB analysis of K-562 using 12276-1-AP

    WB analysis of K-562 using 12276-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12276-1-AP (FIP1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 12276-1-AP

    IF Staining of MCF-7 using 12276-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FIP1L1 antibody (12276-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human colon cancer using 12276-1-AP

    IHC staining of human colon cancer using 12276-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12276-1-AP (FIP1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12276-1-AP

    IHC staining of human colon cancer using 12276-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12276-1-AP (FIP1L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12276-1-AP targets FIP1L1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen FIP1L1 fusion protein Ag2921 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name FIP1 like 1 (S. cerevisiae)
Synonyms Factor interacting with PAP, FIP1, FIP1 like 1 (S. cerevisiae), FIP1 like 1 protein, FIP1L1, FIP1L1 PDGFRalpha, hFip1, Rhe
Calculated Molecular Weight 594 aa, 66 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC017724
Gene Symbol FIP1L1
Gene ID (NCBI) 81608
RRIDAB_2231572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UN15
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FIP1L1 antibody 12276-1-APDownload protocol
IHC protocol for FIP1L1 antibody 12276-1-APDownload protocol
IF protocol for FIP1L1 antibody 12276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols