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FIBP Polyclonal antibody

FIBP Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15968-1-AP

Print datasheet

Synonyms

FGFIBP, FIBP, FIBP 1

验证数据展示

  • WB analysis of BxPC-3 using 15968-1-AP

    WB analysis of BxPC-3 using 15968-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15968-1-AP

    IF Staining of HepG2 using 15968-1-AP

    Immunofluorescent analysis of HepG2 cells using 15968-1-AP (FIBP antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human kidney using 15968-1-AP

    IHC staining of human kidney using 15968-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15968-1-AP (FIBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inBxPC-3 cells, mouse pancreas tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15968-1-AP targets FIBP in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen FIBP fusion protein Ag8743 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibroblast growth factor (acidic) intracellular binding protein
Synonyms FGFIBP, FIBP, FIBP 1
Calculated Molecular Weight 357 aa, 41 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC014388
Gene Symbol FIBP
Gene ID (NCBI) 9158
RRIDAB_2878203
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43427
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FIBP, also named as Acidic fibroblast growth factor intracellular binding protein, is a 364 amino acid protein, which is highly expressed in heart, skeletal muscle and pancreas. FIBP may be involved in mitogenic function of FGF1.

实验方案

Product Specific Protocols
WB protocol for FIBP antibody 15968-1-APDownload protocol
IHC protocol for FIBP antibody 15968-1-APDownload protocol
IF protocol for FIBP antibody 15968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols