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FHL1 Polyclonal antibody

FHL1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 10991-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, zebrafish

应用

WB, IHC, IP, ELISA and More (3)

Four and a half LIM domains protein 1, four and a half LIM domains 1, FLH1A, FHL1B, FHL-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, human brain tissue, mouse colon tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FHL1 Ready-To-Use IHC Kit. FHL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10991-1-AP targets FHL1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP
经测试反应性 human, mouse, rat, zebrafish
文献引用反应性human, mouse, rat, zebrafish
免疫原 FHL1 fusion protein Ag1448 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 four and a half LIM domains 1
别名 Four and a half LIM domains protein 1, four and a half LIM domains 1, FLH1A, FHL1B, FHL-1
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC010998
基因名称 FHL1
Gene ID (NCBI) 2273
RRIDAB_2104230
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13642
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Four-and-a-half LIM domains 1 (FHL1), a member of the FHL protein family, which composed of FHL 1, 2, 3, 4 and ACT (activator of CREM), and characterized by an N-terminal half LIM domain followed by four complete LIM domains. FHL1 may contribute to sarcomere synthesis, assembly and biomechanical stress sensing. FHL1 is also a methylation-silenced tumor-suppressor gene on chromosome X in gastrointestinal cancers, which contributes to the formation of an epigenetic field for cancerization. FHL1(10991-1-AP) is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. This is a rabbit polyantibody raised against full length of human FHL1.

实验方案

Product Specific Protocols
WB protocol for FHL1 antibody 10991-1-APDownload protocol
IHC protocol for FHL1 antibody 10991-1-APDownload protocol
IP protocol for FHL1 antibody 10991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,CoIP,ChIP

J Clin Invest

Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.

Authors - Ding Lihua L
  • KD Validated
humanWB

Genes Dev

Browning of human adipocytes requires KLF11 and reprogramming of PPARγ superenhancers.

Authors - Anne Loft
  • KD Validated
humanWB,IHC

Nat Commun

A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.

Authors - Xiaojie Xu
  • KD Validated

J Cell Biol

Src-mediated phosphorylation converts FHL1 from tumor suppressor to tumor promoter.

Authors - Xiang Wang
humanWB,IHC

Int J Cancer

Downregulation and growth inhibitory role of FHL1 in lung cancer.

Authors - Niu Chang C
human,mouseWB

J Cell Sci

MAL/MRTF-A controls migration of non-invasive cells by upregulation of cytoskeleton-associated proteins.

Authors - Leitner Laura L
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