验证数据展示
经过测试的应用
Positive WB detected in | mouse skeletal muscle tissue, human brain tissue, mouse colon tissue, rat skeletal muscle tissue |
Positive IP detected in | mouse skeletal muscle tissue |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10991-1-AP targets FHL1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
经测试反应性 | human, mouse, rat, zebrafish |
文献引用反应性 | human, mouse, rat, zebrafish |
免疫原 | FHL1 fusion protein Ag1448 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | four and a half LIM domains 1 |
别名 | Four and a half LIM domains protein 1, four and a half LIM domains 1, FLH1A, FHL1B, FHL-1 |
计算分子量 | 32 kDa |
观测分子量 | 32 kDa |
GenBank蛋白编号 | BC010998 |
基因名称 | FHL1 |
Gene ID (NCBI) | 2273 |
RRID | AB_2104230 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q13642 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Four-and-a-half LIM domains 1 (FHL1), a member of the FHL protein family, which composed of FHL 1, 2, 3, 4 and ACT (activator of CREM), and characterized by an N-terminal half LIM domain followed by four complete LIM domains. FHL1 may contribute to sarcomere synthesis, assembly and biomechanical stress sensing. FHL1 is also a methylation-silenced tumor-suppressor gene on chromosome X in gastrointestinal cancers, which contributes to the formation of an epigenetic field for cancerization. FHL1(10991-1-AP) is highly expressed in skeletal muscle and to a lesser extent in heart, placenta, ovary, prostate, testis, small intestine, colon and spleen. This is a rabbit polyantibody raised against full length of human FHL1.
实验方案
Product Specific Protocols | |
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WB protocol for FHL1 antibody 10991-1-AP | Download protocol |
IHC protocol for FHL1 antibody 10991-1-AP | Download protocol |
IP protocol for FHL1 antibody 10991-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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J Clin Invest Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.
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Genes Dev Browning of human adipocytes requires KLF11 and reprogramming of PPARγ superenhancers.
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Nat Commun A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.
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J Cell Biol Src-mediated phosphorylation converts FHL1 from tumor suppressor to tumor promoter. | ||
J Cell Sci MAL/MRTF-A controls migration of non-invasive cells by upregulation of cytoskeleton-associated proteins. |