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验证数据展示

  • WB analysis using 68162-1-Ig

    WB analysis using 68162-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68162-1-Ig (FH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human stomach cancer using 68162-1-Ig

    IHC staining of human stomach cancer using 68162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68162-1-Ig (FH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FH antibody included
  • IHC staining of mouse cerebellum using 68162-1-Ig

    IHC staining of mouse cerebellum using 68162-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68162-1-Ig (FH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FH antibody included
  • WB analysis of HepG2 using 68162-1-Ig

    WB analysis of HepG2 using 68162-1-Ig

    WB result of FH antibody (68162-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells.

  • IHC staining of human placenta using 68162-1-Ig

    IHC staining of human placenta using 68162-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68162-1-Ig (FH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FH antibody included
  • Featured Product
  • KD/KO验证

FH Monoclonal antibody

FH Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68162-1-Ig

产品说明书

CloneNo. 1A9C8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, ELISA

别名

HsFH, Fumarase, EC:4.2.1.2, 1A9C8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, LNCaP cells, HEK-293 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman stomach cancer tissue, human placenta tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FH Ready-To-Use IHC Kit. FH primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68162-1-Ig targets FH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 FH fusion protein Ag30254 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 fumarate hydratase
别名 HsFH, Fumarase, EC:4.2.1.2, 1A9C8
计算分子量 510 aa, 55 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC003108
基因名称 FH
Gene ID (NCBI) 2271
RRIDAB_2923681
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07954
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

实验方案

Product Specific Protocols
WB protocol for FH antibody 68162-1-IgDownload protocol
IHC protocol for FH antibody 68162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols