验证数据展示

WB analysis using 11375-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11375-1-AP
WB result of FH antibody (11375-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells.
IF Staining of HeLa using 11375-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FH antibody (11375-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IP experiment of mouse liver using 11375-1-AP
IP result of anti-FH (IP:11375-1-AP, 4ug; Detection:11375-1-AP 1:8000) with mouse liver tissue lysate 3040 ug.
IHC staining of human placenta using 11375-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with FH antibody included
IHC staining of human placenta using 11375-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with FH antibody included
IHC staining of human normal colon using 11375-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11375-1-AP (FH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with FH antibody included

Featured Product
KD/KO验证

FH Polyclonal antibody

FH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11375-1-AP

产品说明书

引用文献(16)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

HsFH, Fumarase, EC:4.2.1.2

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (16)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293 cells, HepG2 cells, human placenta tissue, Hela cells, Jurkat cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue, L02 cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human placenta tissue, human normal colon, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy FH Ready-To-Use IHC Kit. FH primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 12 publications below
IHC	See 4 publications below
IF	See 1 publications below

产品信息

11375-1-AP targets FH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, caenorhabditis elegans
免疫原	FH fusion protein Ag1934 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	fumarate hydratase
别名	HsFH, Fumarase, EC:4.2.1.2
计算分子量	510 aa, 55 kDa
观测分子量	45-50 kDa
GenBank蛋白编号	BC003108
基因名称	FH
Gene ID (NCBI)	2271
RRID	AB_763171
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P07954
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

实验方案

Product Specific Protocols
WB protocol for FH antibody 11375-1-AP	Download protocol
IHC protocol for FH antibody 11375-1-AP	Download protocol
IF protocol for FH antibody 11375-1-AP	Download protocol
IP protocol for FH antibody 11375-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Authors - Xiaoyu Ma View Article
mouse	WB	Aging Dis Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging Authors - Minghao Yuan View Article
human	WB	Theranostics EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter. Authors - Feng Shi View Article
human	WB	Oncotarget CDC5L drives FAH expression to promote metabolic reprogramming in melanoma. Authors - Zhichao Gu View Article
human	WB	Invest Ophthalmol Vis Sci Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss. Authors - Coupland Sarah E SE View Article
Caenorhabditis elegans	WB	Int J Radiat Biol Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field. Authors - Yongyan Sun View Article

验证数据展示

WB analysis using 11375-1-AP

WB analysis of HepG2 using 11375-1-AP

IF Staining of HeLa using 11375-1-AP

IP experiment of mouse liver using 11375-1-AP

IHC staining of human placenta using 11375-1-AP

IHC staining of human placenta using 11375-1-AP

IHC staining of human normal colon using 11375-1-AP

FH Polyclonal antibody

FH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging

EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter.

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss.

Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.