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验证数据展示

  • WB analysis using 81069-1-RR

    WB analysis using 81069-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81069-1-RR (FGFR4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 81069-1-RR

    IHC staining of mouse lung using 81069-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81069-1-RR (FGFR4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 81069-1-RR

    Affinity and Kinetic Characterization of 81069-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81069-1-RR against Human FGFR4 were performed. The affinity constant is 25 nM.

FGFR4 Recombinant antibody

FGFR4 Recombinant Antibody for WB, IHC, ELISA
Cat No. 81069-1-RR

产品说明书

CloneNo. 5P12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

5P12, CD334, EC:2.7.10.1, FGFR 4, FGFR-4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMOLT-4 cells, Raji cells, Jurkat cells, HeLa cells, HepG2 cells, HT-29 cells, A549 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81069-1-RR targets FGFR4 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 FGFR4 fusion protein Ag31013 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fibroblast growth factor receptor 4
别名 5P12, CD334, EC:2.7.10.1, FGFR 4, FGFR-4
计算分子量 88 kDa
观测分子量100-110 kDa
GenBank蛋白编号BC011847
基因名称 FGFR4
Gene ID (NCBI) 2264
RRIDAB_2918926
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP22455
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FGFR4 antibody 81069-1-RRDownload protocol
IHC protocol for FGFR4 antibody 81069-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols