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验证数据展示

  • IP experiment of SH-SY5Y using 30358-1-AP

    IP experiment of SH-SY5Y using 30358-1-AP

    IP result of anti-FGFR1 (IP:30358-1-AP, 4ug; Detection:30358-1-AP 1:300) with SH-SY5Y cells lysate 770 ug.

  • IHC staining of human colon using 30358-1-AP

    IHC staining of human colon using 30358-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30358-1-AP (FGFR1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 30358-1-AP

    IHC staining of human colon using 30358-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30358-1-AP (FGFR1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 30358-1-AP

    IHC staining of mouse brain using 30358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30358-1-AP (FGFR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat kidney using 30358-1-AP

    IHC staining of rat kidney using 30358-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30358-1-AP (FGFR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 30358-1-AP

    IF Staining of SH-SY5Y using 30358-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using FGFR1 antibody (30358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

FGFR1 Polyclonal antibody

FGFR1 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 30358-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, IP, ELISA

别名

bFGF R 1, BFGFR, bFGF-R-1, CD331, CEK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman colon tissue, mouse brain tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30358-1-AP targets FGFR1 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibroblast growth factor receptor 1
别名 bFGF R 1, BFGFR, bFGF-R-1, CD331, CEK
计算分子量 820 aa, 91 kDa
观测分子量80 kDa, 95 kDa, 105 kDa
GenBank蛋白编号BC018128
基因名称 FGFR1
Gene ID (NCBI) 2260
RRIDAB_3669716
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11362
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processes including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in the FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer, and autosomal dominant Kallmann syndrome. Alternatively spliced variants that encode different protein isoforms have been described.

实验方案

Product Specific Protocols
IHC protocol for FGFR1 antibody 30358-1-APDownload protocol
IF protocol for FGFR1 antibody 30358-1-APDownload protocol
IP protocol for FGFR1 antibody 30358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols