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验证数据展示

  • WB analysis using 24725-1-AP

    WB analysis using 24725-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24725-1-AP (FGFBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 24725-1-AP

    IF Staining of A431 using 24725-1-AP

    Immunofluorescent analysis of A431 cells using 24725-1-AP (FGFBP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

FGFBP3 Polyclonal antibody

FGFBP3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24725-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

C10orf13, FGF binding protein 3, FGF BP3, FGFBP 3, FGFBP3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24725-1-AP targets FGFBP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20573

Product name: Recombinant human FGFBP3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-258 aa of BC025966

Sequence: MTPPKLRASLSPSLLLLLSGCLLAAARREKGAASNVAEPVPGPTGGSSGRFLSPEQHACSWQLLLPAPEAAAGSELALRCQSPDGARHQCAYRGHPERCAAYAARRAHFWKQVLGGLRKKRRPCHDPAPLQARLCAGKKGHGAELRLVPRASPPARPTVAGFAGESKPRARNRGRTRERASGPAAGTPPPQSAPPKENPSERKTNEGKRKAALVPNEERPMGTGPDPDGLDGNAELTETYCAEKWHSLCNFFVNFWNG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibroblast growth factor binding protein 3
别名 C10orf13, FGF binding protein 3, FGF BP3, FGFBP 3, FGFBP3
计算分子量 258 aa, 28 kDa
观测分子量 60-65 kda
GenBank蛋白编号BC025966
基因名称 FGFBP3
Gene ID (NCBI) 143282
RRIDAB_2879691
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAT2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGFBP3 also named as C10orf13 or FGF binding protein 3 is 258 amino acid protein, which belongs to the fibroblast growth factor binding family. FGFBP3 is a secreted protein and may play a role in the regulation of gene transcription. The molecular weight of FGFBP3 is 29kDa, but our results detected the 60-65kDa protein by western blot. We infer that FGFBP3 may exist as dimer.

实验方案

Product Specific Protocols
IF protocol for FGFBP3 antibody 24725-1-APDownload protocol
WB protocol for FGFBP3 antibody 24725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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