验证数据展示
经过测试的应用
| Positive IHC detected in | human stomach tissue, human placenta tissue, human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| IHC | See 6 publications below |
| IF | See 3 publications below |
产品信息
18171-1-AP targets FGF5 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IF, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, goat, rabbit |
| 免疫原 |
CatNo: Ag12824 Product name: Recombinant human FGF5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 16-268 aa of BC074858 Sequence: LSAWAHGEKRLAPKGQPGPAATDRNPRGSSSRQSSSSAMSSSSASSSPAASLGSQGSGLEQSSFQWSPSGRRTGSLYCRVGIGFHLQIYPDGKVNGSHEANMLSVLEIFAVSQGIVGIRGVFSNKFLAMSKKGKLHASAKFTDDCKFRERFQENSYNTYASAIHRTEKTGREWYVALNKRGKAKRGCSPRVKPQHISTHFLPRFKQSEQPELSFTVTVPEKKKPPSPIKPKIPLSAPRKNTNSVKYRLKFRFG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibroblast growth factor 5 |
| 别名 | FGF 5, FGF5, fibroblast growth factor 5, HBGF 5, Smag 82 |
| 计算分子量 | 268 aa, 30 kDa |
| GenBank蛋白编号 | BC074858 |
| 基因名称 | FGF5 |
| Gene ID (NCBI) | 2250 |
| RRID | AB_2878511 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12034 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for FGF5 antibody 18171-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids CRISPR Start-Loss: A Novel and Practical Alternative for Gene Silencing through Base-Editing-Induced Start Codon Mutations.
| ||
iScience Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency | ||
Front Pharmacol Suppression of FGF5 and FGF18 Expression by Cholesterol-Modified siRNAs Promotes Hair Growth in Mice. | ||
Commun Biol Testicular somatic cell-like cells derived from embryonic stem cells induce differentiation of epiblasts into germ cells. | ||
Genes (Basel) CRISPR/Cas9-mediated Disruption of Fibroblast Growth Factor 5 in Rabbits Results in a Systemic Long Hair Phenotype by Prolonging Anagen. |
