FGF-2 Polyclonal antibody

FGF-2 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 11234-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, FC (Intra), ELISA

FGF2, FGF 2, FGF B, FGF basic, FGFB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHEK-293T cells, SKOV-3 cells, HepG2 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue, mouse lung tissue, rat brain tissue, rat kidney tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy FGF2 Ready-To-Use IHC Kit. FGF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11234-1-AP targets FGF-2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibroblast growth factor 2 (basic)
别名 FGF2, FGF 2, FGF B, FGF basic, FGFB
计算分子量 21 kDa
观测分子量31 kDa
GenBank蛋白编号NM_002006
基因名称 FGF2
Gene ID (NCBI) 2247
RRIDAB_2941976
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09038
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

实验方案

Product Specific Protocols
WB protocol for FGF-2 antibody 11234-1-APDownload protocol
IHC protocol for FGF-2 antibody 11234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nat Nanotechnol

Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.

Authors - Xuefei Lu
WB

Biomaterials

Lung endothelial cell-targeted peptide-guided bFGF promotes the regeneration after radiation induced lung injury.

Authors - Dongwei Guan
mouseWB

Diabetes

N 6-Methyladenosine demethylase FTO controls macrophage homeostasis in diabetic vasculopathy

Authors - Siguo Feng
humanWB

BMC Cancer

Multi-omics sequencing revealed endostar combined with cisplatin treated non small cell lung cancer via anti-angiogenesis

Authors - Yufei Wang
mouse,humanWB

Toxicol Appl Pharmacol

Synergistic anticancer effects of ginsenoside CK and gefitinib against gefitinib-resistant NSCLC by regulating the balance of angiogenic factors through HIF-1α/VEGF

Authors - Xiaoping Song
humanWB,IHC

Wound Repair Regen

Negative pressure wound therapy is associated with upregulation of bFGF and ERK1/2 in human diabetic foot wounds.

Authors - Shao-Ling Yang
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