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FGF17 Polyclonal antibody

FGF17 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25314-1-AP

Print datasheet

Synonyms

FGF 13, FGF 17, FGF17, fibroblast growth factor 17

验证数据展示

  • WB analysis of MCF-7 using 25314-1-AP

    WB analysis of MCF-7 using 25314-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25314-1-AP (FGF17 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 25314-1-AP

    IF Staining of NIH/3T3 using 25314-1-AP

    Immunofluorescent analysis of NIH/3T3 cells using 25314-1-AP (FGF17 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, PC-3 cells
Positive IF detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of 25314-1-AP is FGF17 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse
Immunogen FGF17 fusion protein Ag17807 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibroblast growth factor 17
Synonyms FGF 13, FGF 17, FGF17, fibroblast growth factor 17
Calculated Molecular Weight 216 aa, 25 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC105131
Gene Symbol FGF17
Gene ID (NCBI) 8822
RRIDAB_2880025
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60258
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FGF17 antibody 25314-1-APDownload protocol
IF protocol for FGF17 antibody 25314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

Cell

Ex utero monkey embryogenesis from blastocyst to early organogenesis

Authors - Yandong Gong
View Article