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验证数据展示

  • WB analysis using 66811-1-Ig (same clone as 66811-1-PBS)

    WB analysis using 66811-1-Ig (same clone as 66811-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66811-1-Ig (same clone as 66811-1-PBS)

    IHC staining of human liver cancer using 66811-1-Ig (same clone as 66811-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66811-1-Ig (FFAR3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66811-1-Ig (same clone as 66811-1-PBS)

    IF Staining of A549 using 66811-1-Ig (same clone as 66811-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 66811-1-Ig (FFAR3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66811-1-PBS in a different storage buffer formulation.

FFAR3 Monoclonal antibody, PBS Only

FFAR3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66811-1-PBS

产品说明书

CloneNo. 1D10B7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

GPR41, 1D10B7, FFA3R, free fatty acid receptor 3, G protein coupled receptor 41

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66811-1-PBS targets FFAR3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag28025

Product name: Recombinant human FFAR3 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 246-346 aa of BC035657

Sequence: VVGYICGESPAWRIYVTLLSTLNSCVDPFVYYFSSSGFQADFHELLRRLCGLWGQWQQESSMELKEQKGGEEQRADRPAERKTSEHSQGCGTGGQVACAES

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 free fatty acid receptor 3
别名 GPR41, 1D10B7, FFA3R, free fatty acid receptor 3, G protein coupled receptor 41
计算分子量 39 kDa
观测分子量 40-48 kDa
GenBank蛋白编号BC035657
基因名称 FFAR3
Gene ID (NCBI) 2865
RRIDAB_2882154
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14843
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FFAR3 (Free fatty acid receptors 3) also known as GPR41, with another GPR43 protein, both are Gai-coupled receptor activated by short-chain fatty acids (SCFAs) such as acetate, propionate, and butyrate (PMID:26870043). GPR41 protein is translated from the bicistronic mRNA encoding GPR40 and GPR41, where an internal ribosome entry site (IRES) is utilized for the GPR41 coding sequence downstream of GPR40 (PMID:22493486). GPR41 is expressed in adipose tissue, gut, and the peripheral nervous system, and it is involved in SCFA-dependent energy regulation(PMID: 24904531).

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