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验证数据展示

  • WB analysis using 83407-7-RR (same clone as 83407-7-PBS)

    WB analysis using 83407-7-RR (same clone as 83407-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83407-7-RR (FEZ2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83407-7-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83407-7-RR (same clone as 83407-7-PBS)

    IHC staining of human ovary cancer using 83407-7-RR (same clone as 83407-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83407-7-RR (FEZ2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83407-7-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83407-7-RR (same clone as 83407-7-PBS)

    FC experiment of A431 using 83407-7-RR (same clone as 83407-7-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Fez2 Recombinant Antibody (83407-7-RR, Clone:240248B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83407-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83407-7-RR

    Affinity and Kinetic Characterization of 83407-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83407-7-RR against Human FEZ2 were performed. The affinity constant is below 1 nM.

FEZ2 Recombinant antibody, PBS Only

FEZ2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83407-7-PBS

产品说明书

CloneNo. 240248B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

Zygin-2, Zygin II, Zygin 2, HUM3CL, Fasciculation and elongation protein zeta-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83407-7-PBS targets FEZ2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 FEZ2 fusion protein Ag15370 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fasciculation and elongation protein zeta 2 (zygin II)
别名 Zygin-2, Zygin II, Zygin 2, HUM3CL, Fasciculation and elongation protein zeta-2
计算分子量 353 aa, 40 kDa
观测分子量~50 kDa
GenBank蛋白编号BC110386
基因名称 FEZ2
Gene ID (NCBI) 9637
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UHY8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FEZ2 is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles.