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验证数据展示

  • WB analysis using 83407-7-RR (same clone as 83407-7-PBS)

    WB analysis using 83407-7-RR (same clone as 83407-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83407-7-RR (FEZ2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83407-7-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 83407-7-RR (same clone as 83407-7-PBS)

    IHC staining of human ovary cancer using 83407-7-RR (same clone as 83407-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83407-7-RR (FEZ2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83407-7-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83407-7-RR (same clone as 83407-7-PBS)

    FC experiment of A431 using 83407-7-RR (same clone as 83407-7-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Fez2 Recombinant Antibody (83407-7-RR, Clone:240248B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83407-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83407-7-PBS

    Affinity and Kinetic Characterization of 83407-7-PBS

    Biolayer interferometry (BLl) kinetic assay of 83407-7-PBS against Human FEZ2 was performed. The affinity constant is 0.212 nM.

  • Affinity and Kinetic Characterization of 83407-7-RR

    Affinity and Kinetic Characterization of 83407-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83407-7-RR against Human FEZ2 were performed. The affinity constant is below 1 nM.

FEZ2 Recombinant monoclonal antibody, PBS Only

FEZ2 Uni-rAb® Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83407-7-PBS

产品说明书

CloneNo. 240248B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

240248B10, Fasciculation and elongation protein zeta-2, HUM3CL, Zygin 2, Zygin II

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83407-7-PBS targets FEZ2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15370

Product name: Recombinant human FEZ2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-160 aa of BC110386

Sequence: MAADGDWQDFYEFQEPARSLLDQENCNASPEPGAEAGAGAGGGADGFPALACSLEEKLSLCFRPSDPGAEPPRTAVRPITERSLLQGDEIWNALTDNYGNVMPVDWKSSHTRTLHLLTLNLSEKGVSDSLLFDTSDDEELREQLDMHSIIVSCVNDEPLF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fasciculation and elongation protein zeta 2 (zygin II)
别名 240248B10, Fasciculation and elongation protein zeta-2, HUM3CL, Zygin 2, Zygin II
计算分子量 353 aa, 40 kDa
观测分子量~50 kDa
GenBank蛋白编号BC110386
基因名称 FEZ2
Gene ID (NCBI) 9637
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UHY8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FEZ2 is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles.

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