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验证数据展示

  • WB analysis using 67524-1-Ig

    WB analysis using 67524-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67524-1-Ig (FES antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 67524-1-Ig

    IF Staining of A549 using 67524-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 67524-1-Ig (FES antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

FES Monoclonal antibody

FES Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67524-1-Ig

产品说明书

CloneNo. 1E1B11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

1E1B11, EC:2.7.10.2, Feline sarcoma/Fujinami avian sarcoma oncogene homolog, FPS, p93c-fes

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTHP-1 cells, Karpas-299 cells, NR8383 cells, RAW 264.7 cells, TF-1 cells, HL-60 cells, U-937 cells
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67524-1-Ig targets FES in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28108

Product name: Recombinant human FES protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 125-390 aa of BC035357

Sequence: QELTKTHSQDIEKLKSQYRALARDSAQAKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQHHHQLLLPGLLRSLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAAAARIQPEAEYQGFLRQYGSAPDVPPCVTFDESLLEEGEPLEPGELQLNELTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQELRNEEENTHPRERVQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEH

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 feline sarcoma oncogene
别名 1E1B11, EC:2.7.10.2, Feline sarcoma/Fujinami avian sarcoma oncogene homolog, FPS, p93c-fes
计算分子量 822 aa, 94 kDa
观测分子量 93 kDa, 79 kDa, 87 kDa, 85 kDa
GenBank蛋白编号BC035357
基因名称 FES
Gene ID (NCBI) 2242
RRIDAB_2882744
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07332
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for FES antibody 67524-1-IgDownload protocol
WB protocol for FES antibody 67524-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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