Kindlin 3 Polyclonal antibody
Kindlin 3 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, Raji cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
18131-1-AP targets Kindlin 3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Immunogen | Kindlin 3 fusion protein Ag12713 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | fermitin family homolog 3 (Drosophila) |
| Synonyms | Fermitin family homolog 3, FERMT3, KIND3, Kindlin 3, MIG 2, MIG2 like protein, MIG2B, Unc 112 related protein 2, UNC112C, URP2, URP2SF |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 72-76 kDa |
| GenBank Accession Number | BC004347 |
| Gene Symbol | FERMT3 |
| Gene ID (NCBI) | 83706 |
| RRID | AB_2103056 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UX7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kindlin-3 (FERMT-3) is known to be central in hemostasis and thrombosis control and its deficiency disrupts platelet aggregation and causes Leukocyte Adhesion Deficiency disease. Point mutations in the kindlin-3 gene have been identified in humans with a rare inherited Integrin Activation efficiency Disease (IADD), also designated as Leukocyte Adhesion Deficiency syndrome (LAD-III or LADI variant). Kindlin-3 expression is restricted to cells of hematopoietic origin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Kindlin 3 antibody 18131-1-AP | Download protocol |
| IHC protocol for Kindlin 3 antibody 18131-1-AP | Download protocol |
| IF protocol for Kindlin 3 antibody 18131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Biol Non-muscle myosins control radial glial basal endfeet to mediate interneuron organization |
