验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11453-1-AP targets Kindlin 2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, zebrafish, hamster |
| 免疫原 | Kindlin 2 fusion protein Ag2013 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fermitin family homolog 2 (Drosophila) |
| 别名 | Fermitin family homolog 2, FERMT2, KIND2, Kindlin 2, mig 2, MIG2, PLEKHC1, UNC112, UNC112B |
| 计算分子量 | 680 aa, 78 kDa |
| 观测分子量 | 78 kDa |
| GenBank蛋白编号 | BC017327 |
| 基因名称 | Kindlin 2 |
| Gene ID (NCBI) | 10979 |
| RRID | AB_2262660 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96AC1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FERMT2, also named as KIND2, MIG2, PLEKHC1, MIG-2 and Kindlin-2, belongs to the kindlin family. There are three FERMT2 isoforms that participate in the connection between extracellular matrix (ECM) adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. FERMT2 recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Variable expression in human cancers and mesenchymal cancer invasion are now linked with FERMT2, which may plays important role in carcinormas development. Catalog#11453-1-AP is a rabbit polyclonal antibody against human FERMT2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Kindlin 2 antibody 11453-1-AP | Download protocol |
| IHC protocol for Kindlin 2 antibody 11453-1-AP | Download protocol |
| IP protocol for Kindlin 2 antibody 11453-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth. | ||
Circ Res Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.
| ||
Cancer Res Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis | ||
J Am Soc Nephrol Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.
| ||
J Cell Sci Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.
| ||
J Cell Sci Kindlin-2 promotes rear focal adhesion disassembly and directional persistence in cell migration
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