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Host
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验证数据展示

  • WB analysis of HepG2 using 83536-1-RR (same clone as 83536-1-PBS)

    WB analysis of HepG2 using 83536-1-RR (same clone as 83536-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83536-1-RR (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83536-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83536-1-RR (same clone as 83536-1-PBS)

    FC experiment of U2OS using 83536-1-RR (same clone as 83536-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Fem1c Recombinant Antibody (83536-1-RR, Clone:240126G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83536-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83536-1-RR

    Affinity and Kinetic Characterization of 83536-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83536-1-RR against Human FEM1C were performed. The affinity constant is 0.896 nM.

FEM1C Recombinant antibody, PBS Only

FEM1C Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83536-1-PBS

产品说明书

CloneNo. 240126G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

FEM1-gamma, FEM1A, FEM1 gamma, FEM1, FEM

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83536-1-PBS targets FEM1C in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 FEM1C fusion protein Ag5099 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fem-1 homolog c (C. elegans)
别名 FEM1-gamma, FEM1A, FEM1 gamma, FEM1, FEM
计算分子量 69 kDa
观测分子量 69 kDa
GenBank蛋白编号BC028369
基因名称 FEM1C
Gene ID (NCBI) 56929
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96JP0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.