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验证数据展示

  • IF Staining of PC-3 using 83810-1-RR

    IF Staining of PC-3 using 83810-1-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using FEM1B antibody (83810-1-RR, Clone: 240607D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A431 using 83810-1-RR

    FC experiment of A431 using 83810-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug FEM1B Recombinant antibody (83810-1-RR, Clone:240607D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of PC-3 using 83810-1-RR

    IF Staining of PC-3 using 83810-1-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using FEM1B antibody (83810-1-RR, Clone: 240607D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FEM1B Recombinant monoclonal antibody

FEM1B Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83810-1-RR

产品说明书

CloneNo. 240607D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

FEM1 beta, fem 1 homolog b (C. elegans), F1A-alpha, F1AA, F1A alpha

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy® FEM1B Ready-To-Use IHC Kit. FEM1B primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83810-1-RR targets FEM1B in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fem-1 homolog b (C. elegans)
别名 FEM1 beta, fem 1 homolog b (C. elegans), F1A-alpha, F1AA, F1A alpha
计算分子量 70 kDa
GenBank蛋白编号NM_015322
基因名称 FEM1B
Gene ID (NCBI) 10116
RRIDAB_3671398
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UK73
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FEM1B, also named F1AA and KIAA0396, belongs to the fem-1 family. FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. It is involved in glucose homeostasis in pancreatic islets.

实验方案

Product Specific Protocols
FC protocol for FEM1B antibody 83810-1-RRDownload protocol
IF protocol for FEM1B antibody 83810-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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