验证数据展示
经过测试的应用
| Positive WB detected in | mouse lung tissue, rat testis tissue, mouse testis tissue, rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34182-1-AP targets FDX1 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Ag41687 Product name: Recombinant mouse FDX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 64-184 aa of BC017063 Sequence: DKITVHFINRDGETLTTKGKVGDSLLDVVVENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAYGLTDRSRLGCQICLTKSMDNMTVRVPETVADARQSIDVGKTS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ferredoxin 1 |
| 别名 | Adrenal ferredoxin, Adrenodoxin, mitochondrial, ADX, FDX, Ferredoxin-1 |
| 计算分子量 | 184 aa, 19 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC017063 |
| 基因名称 | FDX1 |
| Gene ID (NCBI) | 14148 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P46656 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ferredoxin 1 (FDX1) is a small mitochondrial protein and recent studies have shown that FDX1 plays an important role in tumor cuproptosis. FDX1 expression is significantly downregulated in HCC tissues. FDX1 downregulation promotes HCC cell proliferation, invasion in vitro and growth, metastasis in vivo. In addition, FDX1 affects metabolism of HCC cells and is associated with autophagy (PMID: 39128228). FDX1 is localized to the mitochondria and contains a 64-amino-acid mitochondrial transit peptide that is cleaved during post-translational processing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FDX1 antibody 34182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
