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验证数据展示

  • WB analysis using 12592-1-AP

    WB analysis using 12592-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12592-1-AP (FDX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 12592-1-AP

    WB analysis of HEK-293 using 12592-1-AP

    WB result of FDX1 antibody (12592-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDX1 transfected HEK-293 cells.

  • WB analysis of HepG2 using 12592-1-AP

    WB analysis of HepG2 using 12592-1-AP

    WB result of FDX1 antibody (12592-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDX1 transfected HepG2 cells.

  • IHC staining of human kidney using 12592-1-AP

    IHC staining of human kidney using 12592-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FDX1 antibody included
  • IHC staining of human kidney using 12592-1-AP

    IHC staining of human kidney using 12592-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FDX1 antibody included
  • IHC staining of human colon cancer using 12592-1-AP

    IHC staining of human colon cancer using 12592-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FDX1 antibody included
  • IHC staining of human kidney using 12592-1-AP

    IHC staining of human kidney using 12592-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FDX1 antibody included
  • IHC staining of human placenta using 12592-1-AP

    IHC staining of human placenta using 12592-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FDX1 antibody included
  • IHC staining of mouse kidney using 12592-1-AP

    IHC staining of mouse kidney using 12592-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FDX1 antibody included
  • IHC staining of rat adrenal gland using 12592-1-AP

    IHC staining of rat adrenal gland using 12592-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FDX1 antibody included
  • IHC staining of human colon cancer using 12592-1-AP

    IHC staining of human colon cancer using 12592-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FDX1 antibody included
  • IF Staining of HepG2 using 12592-1-AP

    IF Staining of HepG2 using 12592-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDX1 antibody (12592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

FDX1 Polyclonal antibody

FDX1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12592-1-AP

产品说明书

引用文献(252)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (3)

别名

ADX, FDX, Ferredoxin-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HepG2 cells, PC-3 cells, mouse testis tissue
Positive IHC detected inhuman kidney tissue, human liver tissue, human ovary tumor tissue, rat adrenal gland tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy® FDX1 Ready-To-Use IHC Kit. FDX1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12592-1-AP targets FDX1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, sheep
免疫原

CatNo: Ag3301

Product name: Recombinant human FDX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-184 aa of BC017063

Sequence: MAAAGGARLLRAASAVLGGPAGRWLHHAGSRAGSSGLLRNRGPGGSAEASRSLSVSARARSSSEDKITVHFINRDGETLTTKGKVGDSLLDVVVENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAYGLTDRSRLGCQICLTKSMDNMTVRVPETVADARQSIDVGKTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ferredoxin 1
别名 ADX, FDX, Ferredoxin-1
计算分子量 184 aa, 19 kDa
观测分子量 14 kDa
GenBank蛋白编号BC017063
基因名称 FDX1
Gene ID (NCBI) 2230
RRIDAB_11182486
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10109
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.

实验方案

Product Specific Protocols
IF protocol for FDX1 antibody 12592-1-APDownload protocol
IHC protocol for FDX1 antibody 12592-1-APDownload protocol
WB protocol for FDX1 antibody 12592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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Radiotherapy promotes cuproptosis and synergizes with cuproptosis inducers to overcome tumor radioresistance

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SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.

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humanWB

Imeta

Clinical features and molecular landscape of cuproptosis signature-related molecular subtype in gastric cancer

Authors - Wei Chong
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mouseWB

Adv Mater

Multifunctional Copper-Phenolic Nanopills Achieve Comprehensive Polyamines Depletion to Provoke Enhanced Pyroptosis and Cuproptosis for Cancer Immunotherapy

Authors - Guoqing Zhu
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mouseWB

Adv Mater

One-Step Symbiosis of Bimetallic Peroxides Nanoparticles to Induce Ferroptosis/Cuproptosis and Activate cGAS-STING Pathway for Enhanced Tumor Immunotherapy

Authors - Bin Liu
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