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- KD/KO Validated
FDX1 Polyclonal antibody
FDX1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, HepG2 cells, PC-3 cells, mouse testis tissue |
| Positive IHC detected in | human kidney tissue, human liver tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12592-1-AP targets FDX1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, sheep |
| Immunogen | FDX1 fusion protein Ag3301 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ferredoxin 1 |
| Synonyms | Adrenal ferredoxin, Adrenodoxin, mitochondrial, ADX, FDX, FDX1, ferredoxin 1, Hepatoredoxin, LOH11CR1D |
| Calculated Molecular Weight | 184 aa, 19 kDa |
| Observed Molecular Weight | 14 kDa |
| GenBank Accession Number | BC017063 |
| Gene Symbol | FDX1 |
| Gene ID (NCBI) | 2230 |
| RRID | AB_11182486 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10109 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FDX1 antibody 12592-1-AP | Download protocol |
| IHC protocol for FDX1 antibody 12592-1-AP | Download protocol |
| IF protocol for FDX1 antibody 12592-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation. | ||
Nat Chem Biol Mitochondrial metabolism promotes adaptation to proteotoxic stress.
| ||
J Biol Chem Lipoylation is dependent on the ferredoxin FDX1 and dispensable under hypoxia in human cells | ||
Front Oncol ITGB1-mediated molecular landscape and cuproptosis phenotype induced the worse prognosis in diffuse gastric cancer | ||
Heliyon Evaluation the role of cuproptosis-related genes in the pathogenesis, diagnosis and molecular subtypes identification of atherosclerosis |
