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验证数据展示

  • IHC staining of human tonsillitis using 26767-1-AP

    IHC staining of human tonsillitis using 26767-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26767-1-AP (FCHO1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 26767-1-AP

    IHC staining of human tonsillitis using 26767-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26767-1-AP (FCHO1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Raji using 26767-1-AP

    WB analysis of Raji using 26767-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 26767-1-AP (FCHO1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

FCHO1 Polyclonal antibody

FCHO1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26767-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KIAA0290, F-BAR domain only protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26767-1-AP targets FCHO1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25291

Product name: Recombinant human FCHO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 45-180 aa of BC028021

Sequence: ETYSKAMAKLSKLASNGTPMGTFAPLWEVFRVSSDKLALCHLELTRKLQDLIKDVLRYGEEQLKTHKKCKEEVVSTLDAVQVLSGVSQLLPKSRENYLNRCMDQERLRRESTSQKEMDKAETKTKKAAESLRRSVE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FCH domain only 1
别名 KIAA0290, F-BAR domain only protein 1
计算分子量 97 kDa
观测分子量 110 kDa
GenBank蛋白编号BC028021
基因名称 FCHO1
Gene ID (NCBI) 23149
RRIDAB_2880626
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14526
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FCHO1 is composed of 889 amino acid residues and contains an N-terminal FES-CIP4 homology (FCH) domain and an evolutionarily conserved C-terminal FCHO homology (FOH) domain. FCHO1 functions in an early step of clathrin-mediated endocytosis. FCHO1 is required for plasma membrane clathrin-coated vesicle (CCV) budding and marked sites of CCV formation (PMID: 20448150).

实验方案

Product Specific Protocols
IHC protocol for FCHO1 antibody 26767-1-APDownload protocol
WB protocol for FCHO1 antibody 26767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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