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FCGR2B / CD32b Polyclonal antibody

FCGR2B / CD32b Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21541-1-AP

Print datasheet

Synonyms

CD32, CD32B, CDw32, Fc gamma RII b, Fc gamma RIIb, FCG2, FCGR2, FCGR2B, FcRII b, IGFR2, IgG Fc receptor II b

验证数据展示

  • WB analysis of human placenta using 21541-1-AP

    WB analysis of human placenta using 21541-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 21541-1-AP

    IHC staining of human placenta using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with FCGR2B antibody included
  • IHC staining of human placenta using 21541-1-AP

    IHC staining of human placenta using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with FCGR2B antibody included

经过测试的应用

Positive WB detected inhuman placenta tissue, Raji cells
Positive IHC detected inhuman placenta tissue, human spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FCGR2B Ready-To-Use IHC Kit. FCGR2B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21541-1-AP is FCGR2B / CD32b Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human
Immunogen FCGR2B / CD32b fusion protein Ag16189 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fc fragment of IgG, low affinity IIb, receptor (CD32)
Synonyms CD32, CD32B, CDw32, Fc gamma RII b, Fc gamma RIIb, FCG2, FCGR2, FCGR2B, FcRII b, IGFR2, IgG Fc receptor II b
Calculated Molecular Weight 310 aa, 34 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC031992
Gene Symbol FCGR2B
Gene ID (NCBI) 2213
RRIDAB_2878881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31994
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FCGR2B / CD32b antibody 21541-1-APDownload protocol
IHC protocol for FCGR2B / CD32b antibody 21541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols