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验证数据展示

  • WB analysis of human placenta using 21541-1-AP

    WB analysis of human placenta using 21541-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21541-1-AP (FCGR2B / CD32b antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 21541-1-AP

    IHC staining of human tonsillitis using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21541-1-AP (FCGR2B / CD32b antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FCGR2B antibody included
  • IHC staining of human tonsillitis using 21541-1-AP

    IHC staining of human tonsillitis using 21541-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21541-1-AP (FCGR2B / CD32b antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FCGR2B antibody included

FCGR2B / CD32b Polyclonal antibody

FCGR2B / CD32b Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21541-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

FCGR2B, Fc-gamma RII-b, FCG2, Fc gamma RIIb, Fc gamma RII b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, Raji cells
Positive IHC detected inhuman tonsillitis tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FCGR2B Ready-To-Use IHC Kit. FCGR2B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21541-1-AP targets FCGR2B / CD32b in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 FCGR2B / CD32b fusion protein Ag16189 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIb, receptor (CD32)
别名 FCGR2B, Fc-gamma RII-b, FCG2, Fc gamma RIIb, Fc gamma RII b
计算分子量 310 aa, 34 kDa
观测分子量 36 kDa
GenBank蛋白编号BC031992
基因名称 CD32b
Gene ID (NCBI) 2213
RRIDAB_2878881
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31994
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FCGR2B / CD32b antibody 21541-1-APDownload protocol
IHC protocol for FCGR2B / CD32b antibody 21541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Mol Ther

High-dose systemic adeno-associated virus vector administration causes liver and sinusoidal endothelial cell injury

Authors - Juliette Hordeaux
View Article