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验证数据展示

  • WB analysis using 27138-1-AP

    WB analysis using 27138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27138-1-AP (FCGR2A / CD32a antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 27138-1-AP

    IHC staining of human tonsillitis using 27138-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27138-1-AP (FCGR2A / CD32a antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FCGR2A/CD32A antibody included
  • IHC staining of human tonsillitis using 27138-1-AP

    IHC staining of human tonsillitis using 27138-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27138-1-AP (FCGR2A / CD32a antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FCGR2A/CD32A antibody included

FCGR2A / CD32a Polyclonal antibody

FCGR2A / CD32a Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27138-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CD32, Fc-gamma RII-a, FCG2, Fc gamma RIIa, Fc gamma RII a

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU-937 cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy FCGR2A/CD32A Ready-To-Use IHC Kit. FCGR2A/CD32A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27138-1-AP targets FCGR2A / CD32a in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25878

Product name: Recombinant human FCGR2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 44-215 aa of BC020823

Sequence: LEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQKFSHLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIa, receptor (CD32)
别名 CD32, Fc-gamma RII-a, FCG2, Fc gamma RIIa, Fc gamma RII a
计算分子量 35 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC020823
基因名称 CD32a
Gene ID (NCBI) 2212
RRIDAB_2880770
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12318
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

实验方案

Product Specific Protocols
IHC protocol for FCGR2A / CD32a antibody 27138-1-APDownload protocol
WB protocol for FCGR2A / CD32a antibody 27138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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