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验证数据展示

  • WB analysis using 20464-1-AP

    WB analysis using 20464-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20464-1-AP (FBXW9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 20464-1-AP

    IF Staining of U2OS using 20464-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using FBXW9 antibody (20464-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

FBXW9 Polyclonal antibody

FBXW9 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20464-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Fbw9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, Jurkat cells, LNCaP cells, MOLT-4 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20464-1-AP targets FBXW9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 FBXW9 fusion protein Ag14300 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F-box and WD repeat domain containing 9
别名 Fbw9
计算分子量 488 aa, 54 kDa
观测分子量50-54 kDa
GenBank蛋白编号BC004290
基因名称 FBXW9
Gene ID (NCBI) 84261
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5XUX1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FBXW9 antibody 20464-1-APDownload protocol
IF protocol for FBXW9 antibody 20464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols