验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, MDA-MB-231 cell, U-87 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33578-1-AP targets FBXW8 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36986 Product name: Recombinant human FBXW8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-303 aa of BC037296 Sequence: MDDYSLDEFRRRWQEELAQAQAPKKRRRPEAAERRARRPEVGSGRSEQASGDPALAQGLLEGAGRPPAARATRAEGQDVASRSRSPLAREGAGGGEQLVDQLIRDLNEMNDVPFFDIQLPYELAINIFQYLDRKELGRCAQVSKTWKVIAEDEVLWYRLCQQEGHLPDSSISDYSCWKLIFQECRAKEHMLQTNWKNRKGAVSELEHVPDTVLCDVHSHDGVVIAGYTSGDVRVWDTRTWDYVAPFLESEDEEDEPGMQPNVSFVRINSSLAVAAYEDGFLNIWDLRTGKYPVHRFEHDARIQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | F-box and WD repeat domain containing 8 |
| 别名 | F-box and WD-40 domain-containing protein 8, F-box only protein 29, F-box/WD repeat-containing protein 8, FBW6, FBW8 |
| 计算分子量 | 67 kDa |
| 观测分子量 | 67 kDa, 60 kDa |
| GenBank蛋白编号 | BC037296 |
| 基因名称 | FBXW8 |
| Gene ID (NCBI) | 26259 |
| RRID | AB_3743018 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8N3Y1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fbxw8 (also known as Fbw6 or Fbx29) is an F-box protein that is assembled with Cul7 in an SCF-like E3 complex. FBXW8 directs the proteasomal degradation of β-TrCP1 in MAPK pathway-dependent manner, and the attenuation of β-TrCP1 by FBXW8 is important for Cdc25A-mediated cell cycle transition from G1 phase to S phase as well as DNA damage-free progression of S phase (PMID: 33742524).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXW8 antibody 33578-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
